HPTLC method development and validation for the simultaneous determination of preserpine and gallic acid in and ayurvedic formulation
By: Adhyapak, A
.
Contributor(s): Jadhav, A. P.
Publisher: Mumbai Indian Drug Manufacture's Association - IDMA 2019Edition: Vol.56(9), Sep.Description: 55-60p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian drugsSummary: A simple, precise, accurate, robust HPTLC method was developed and validated for simultaneous estimation of reserpine and gallic acid in an Ayurvedic formulation. The two markers were resolved using TLC plates pre-coated with silica gel 60F254 using the mobile phase Toluene: Acetone: Glacial acetic acid in the ratio 4:1:1 v/v with a saturation time of 10 minutes. The Rf values of gallic acid and reserpine were found to be 0.31 ± 0.2 and 0.54 ± 0.2, respectively at 268 nm. Linear response was observed in the concentration range of 20–80 ppm for reserpine and 150–210 ppm for gallic acid, with correlation coefficient (r2 ) of 0.995 and 0.997 for reserpine and gallic acid respectively. The developed method was applied for the quantitation of markers in a marketed formulation, Cardostab tablet. This method can also be used to evaluate other formulations containing reserpine and gallic acid as the constituents, thus conforming to the need of ensuring quality and safety of polyherbal formulations.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020031 |
A simple, precise, accurate, robust HPTLC method was developed and validated for simultaneous estimation of reserpine and gallic acid in an Ayurvedic formulation. The two markers were resolved using TLC plates pre-coated with silica gel 60F254 using the mobile phase Toluene: Acetone: Glacial acetic acid in the ratio 4:1:1 v/v with a saturation time of 10 minutes. The Rf values of gallic acid and reserpine were found to be 0.31 ± 0.2 and 0.54 ± 0.2, respectively at 268 nm. Linear response was observed in the concentration range of 20–80 ppm for reserpine and 150–210 ppm for gallic acid, with correlation coefficient (r2 ) of 0.995 and 0.997 for reserpine and gallic acid respectively. The developed method was applied for the quantitation of markers in a marketed formulation, Cardostab tablet. This method can also be used to evaluate other formulations containing reserpine and gallic acid as the constituents, thus conforming to the need of ensuring quality and safety of polyherbal formulations.
Click on an image to view it in the image viewer
There are no comments for this item.